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N&B data fitting. From the image stack of HeLa cells transfected with DNA plasmid encoding for monomeric sGFP2 (A) the brightness image is created (B) by dividing the intensity variance by the average for each pixel. For moving cells, an artificial high brightness will be obtained in the pixels at the edges of the cell (C). The scalebar corresponds to 5 μm.

Journal: FEBS Open Bio

Article Title: Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

doi: 10.1002/2211-5463.70348

Figure Lengend Snippet: N&B data fitting. From the image stack of HeLa cells transfected with DNA plasmid encoding for monomeric sGFP2 (A) the brightness image is created (B) by dividing the intensity variance by the average for each pixel. For moving cells, an artificial high brightness will be obtained in the pixels at the edges of the cell (C). The scalebar corresponds to 5 μm.

Article Snippet: HeLa cell culture (ATCC, Manassas, VA, USA), (RRID:CVCL_0030).

Techniques: Transfection, Plasmid Preparation